Multiphoton imaging is a technique used in biological and medical research to visualize and study tissues at a cellular level. It involves using multiple photons of light to excite fluorescent molecules within the tissue, allowing for high-resolution, deep-tissue imaging with minimal damage to the sample. This imaging technique is particularly useful for studying live cells and tissues as it offers better penetration and reduced phototoxicity compared to other imaging methods. Multiphoton imaging has applications in various fields, including neuroscience, cancer research, and developmental biology.